Gyrase





Gyrase (Gyr) is a type of topoisomerase II in prokaryotes which unwinds double stranded DNA. The DNA Gyr cutting allows the formation of a negative DNA supercoil which enables replication of DNA. Gyr consists of 2 subunits: GyrA and GyrB. Reverse gyrase (Top-RG) is a type of topoisomerase I which catalyses the formation of positive DNA supercoil.

Gyrase Subunit A
3l6v – GyrA C-terminal – Xanthomonas campestris

2wl2, 2y3p – EcGyrA N-terminal+simocylinone – Escherichia coli

1ajb - EcGyrA N-terminal+novobiocin

1zi0, 1ab4 - EcGyrA C-terminal

3ku8 – EcGyrA fragment+CcdB

1x75 – EcGyrA14+CcdB

3kua - GyrA fragment+CcdB – Vibrio fischeri

3ilw, 3ifz - MtGyrA N-terminal – Mycobacterium tuberculosis

1suu - GyrA C-terminal – Borrelia burgdorferi

3no0 - GyrA C-terminal – Aquifex aeolicus

3lpx – GyrA N-terminal – Colwellia psychrerithraea

Gyrase Subunit B
3g75, 3g7b, 3g7e – GyrB+thiazole inhibitor – Staphylococcus aureus

2zjt, 3ig0, 3m4i - MtGyrB C-terminal

3cwv – GyrB truncated – Myxococcus xanthus

1kzn, 1ei1 - EcGyrB N-terminal+clorobiocin

1aj6 - EcGyrB N-terminal+novobiocin

1kij – GyrB domain+novobiocin – Thermus thermophilus

Gyrase Subunit A+Subunit B
2xco, 2xcq - SaGyrB C-terminal-SaGyrA N-terminal fusion

2xcr, 2xcs - SaGyrB C-terminal-SaGyrA N-terminal fusion (mutant)+DNA

2xct - SaGyrB C-terminal-SaGyrA N-terminal fusion (mutant) +DNA+ ciprofloxacin

3nuh – EcGyrA+EcGyrB

Reverse Gyrase
1gku – AfTop-RG – Archaeoglobus fulgidus

1gl9 - AfTop-RG+ADPNP

3oiy – Top-RG helicase domain – Thermotoga maritima

Additional Resources
For additional information, see: Bacterial Infections